McCoy’S 5A Cell Culture Medium With High Concentration Glucose FDA

Cell Culture Medium

McCoy’s 5A medium Modified Basal Medium 5A medium with high concentration of glucose

product description:

McCoy’s 5A medium is a medium designed by Thomas McCoy et al. in 1959, and it was originally specially used for the cultivation of Novikoff Hepatoma cells. McCoy’s 5A medium is a modified Basal Medium 5A. Unlike other media, McCoy’s 5A contains reduced glutathione, back to-peptone, and high concentrations of glucose.

Product Usage:

McCoy’s 5A is widely used in the culture of various types of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum, etc. In addition, McCoy’s 5A medium is also used for tissue biopsy culture, cell line establishment, and the culture of some lymphocytes and difficult-to-culture cells...

For a wide range of cell culture applications, we offer McCoy’s 5A Modified Medium in various components.

L-glutamine (Glutamine) is an essential nutrient element in cell culture, but it is unstable in solution and will spontaneously degrade. The medium without L-glutamine can be adjusted arbitrarily according to research needs. - The amount of glutamine, adding fresh L-glutamine or its substitutes is more conducive to the growth of cells when using.

Phenol red is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Penicillin-streptomycin mixed solution is usually also called "double antibody", which is the most commonly used antibiotic to prevent microbial contamination in vitro culture. Among them, penicillin can interfere with the synthesis of bacterial cell walls and is particularly effective against Gram-positive bacteria; while streptomycin Mycin can bind to the 30S subunit of bacterial ribosomes and inhibit the synthesis of bacterial proteins. It is effective against both Gram-negative bacteria and Gram-positive bacteria but is particularly effective against Gram-negative bacteria. The combination of penicillin and streptomycin can prevent most bacterial contamination.

This product contains amino acids, vitamins, inorganic salts, and other ingredients required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product should be used with serum or serum-free supplements.

Precautions:

This product is sterilized by filtration, and aseptic operation should be paid attention to when using it to avoid contamination;

In order to maintain the best use effect of this product, do not freeze or thaw;

This product is only used for scientific research or further research, not for diagnosis and treatment.