MEM Cell Culture Medium 500ml Light Red Minimum Essential Medium

Cell Culture Medium

MEM Medium Minimum Essential Medium Cell Culture Medium Minimum Essential Medium

product description:

MEM medium only contains 12 kinds of essential amino acids, glutamine, and 8 kinds of vitamins. It has simple components and is mainly used for the culture of adherent cells. It can also be used for other types of cell culture after the formula is modified.

Product Usage:

MEM medium (Minimum Essential Medium), also known as minimum essential medium, minimum essential medium, or low-volume Eagle medium, was developed by Harry Eagle on the basis of Eagle Basic Medium (BEM), and is the most basic, medium with the widest range of application, is one of the most commonly used media in animal cell culture, including HeLa, BHK-21, 293, HT-1080, MCF-7, fibroblast, and primary Rat astrocytes, etc.

For a wide range of cell culture applications, we offer modified MEM media in a variety of components.

Description of product ingredients:

L-glutamine (Glutamine)

It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to the growth of cells.

NEAA (non-essential amino acids)

On the basis of MEM medium, add L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine, and Glycine contains 7 kinds of NEAA, which can reduce the side effects of non-essential amino acids produced by cells themselves during cell culture, and effectively promote cell proliferation and metabolism.

glucose

The concentration of glucose can be adjusted at will according to the needs of the research, which is convenient and quick. High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells.

Phenol red

is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.