500ml Modified MEM Alpha Medium Fluid Deficient Cell Selection

500ml Modified MEM Alpha Medium Fluid Deficient Cell Selection

MEM α medium Modified MEM medium Cell culture fluid-deficient cell selection medium

product description:

MEM α is an improved MEM medium. Compared with MEM medium, MEM α medium is more nutritious. On the basis of MEM, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin, ascorbic acid, etc. are added. Element

Product Usage:

Widely used in the culture of various mammalian suspension and adherent cells. MEM α medium without nucleosides and deoxynucleosides is often used as a selective medium for DG44 and other DHFR-deficient cells.

For a wide range of cell culture applications, we offer MEM α-modified media in a variety of components.

Product ingredients:

L-glutamine (Glutamine)

It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to the growth of cells.

L-Alanyl-L-glutamine (Alanyl-glutamine, Ala-Glu)

Also known as alanyl-glutamine and pro glutamine, it is an advanced cell culture additive that can directly replace L-glutamine in cell culture medium. L-glutamine (Glutamine) is an essential nutrient in cell culture, but it is unstable in solution and will spontaneously degrade to generate ammonia and pyroglutamic acid, of which ammonia is harmful to cells; and L-alanyl -L-Glutamine is very stable in aqueous solution and will not degrade spontaneously. The mechanism used by cells is: during cell culture, cells will gradually release a peptidase into the culture medium to hydrolyze L-alanyl-L-glutamyl into L-alanine and L-glutamine, Cells then take up these two hydrolysates. The process of cell utilization of L-alanyl-L-glutamine is similar to the fed-batch culture strategy, in which low-concentration levels of L-glutamine are continuously added to the culture medium, thereby improving the utilization of L-glutamine rate, and will not generate excess ammonia, which is more conducive to cell growth. L-alanyl-L-glutamyl can replace equimolar L-glutamine, is suitable for all cells, has almost no adaptation, and can prolong the culture time of cells and reduce the number of passages, which saves time and money. Activity decreased more slowly compared to cells cultured in a medium supplemented with L-glutamine. The slightly longer lag period is due to the time required for the release of the peptidase and the digestion of the dipeptide.

Phenol red:

It is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Five advantages of iclean cell culture medium:

1. Complete products, 280 kinds of basic culture media

2. More than 200 kinds of special media with missing components or additional components are available in stock

3. The medium can be customized according to the needs, and the composition/concentration/ph value can be customized, etc.

4. The performance of the product is stable, and it is produced by an automatic filling line, and the difference between product batches is small

5. Strict quality control system, sterility testing, mycoplasma, endotoxin testing, cell growth experiment, light inspection, etc.