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Disposable DNA Extraction Kits PCR Viral Nucleic Acid Extraction Kit

Disposable DNA Extraction Kits PCR Viral Nucleic Acid Extraction Kit

product description:

HUACHENYANG DNA Extraction Kit provides a fast and efficient magnetic bead method to purify and extract DNA (including genomic, mitochondrial and viral DNA) from preserved tissues, saliva, body fluids, as well as from oral cavity, cervix, skin cells, bacterial cells, etc. Biological samples can be stored at room temperature for up to 30 days in our proprietary storage buffer before processing without significant loss of DNA yield or quality (more than 30 days if stored frozen) No phenol/ Chloroform extraction or alcohol precipitation yields high-quality genomic DNA within 15 minutes, with an average DNA yield of 8 μg per buccal swab. Purified DNA, approximately 20-30 kb, suitable for downstream applications such as PCR or other enzymatic reactions

 

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Product Details:

Place of Origin: China
Brand Name: HUACHENYANG
Certification: ISO13485,NMPA,

Disposable DNA Extraction Kits PCR Viral Nucleic Acid Extraction Kit

Description

Model: CY-F006-22 (200 Preps-saliva) Reagent Bottle Size/volume1: CY1: 60mL
Reagent Bottle Size/volume2: CY2: 125mL Reagent Bottle Size/volume3: CY3: 125mL
Reagent Bottle Size/volume4: CY4: 125mL Reagent Bottle Size/volume5: CY5: 15mL
High Light:

Disposable DNA Extraction Kits

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125mL DNA Extraction Kits

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Viral Nucleic Acid Extraction Kit

 

Disposable DNA Extraction Kits PCR Viral Nucleic Acid Extraction Kit

 

RNA Laboratory Detection Kit Disposable PCR Virus Nucleic Acid Extraction Kit Nucleic Acid Purification Extraction Kit

 

product description:

HUACHENYANG DNA Extraction Kit provides a fast and efficient magnetic bead method to purify and extract DNA (including genomic, mitochondrial and viral DNA) from preserved tissues, saliva, body fluids, as well as from oral cavity, cervix, skin cells, bacterial cells, etc. Biological samples can be stored at room temperature for up to 30 days in our proprietary storage buffer before processing without significant loss of DNA yield or quality (more than 30 days if stored frozen) No phenol/ Chloroform extraction or alcohol precipitation yields high-quality genomic DNA within 15 minutes, with an average DNA yield of 8 μg per buccal swab. Purified DNA, approximately 20-30 kb, suitable for downstream applications such as PCR or other enzymatic reactions

 

Description:

 

The main components are magnetic beads, proteinase K, sodium chloride. Magnesium Chloride. Potassium chloride, Tris, etc.

 

feature:

Efficient, monospecific extraction of DNA to maximize removal of impurity proteins and other organic compounds from cells. The extracted DNA has large fragments, high purity, and stable and reliable quality.

 

model CY-F006-22 (200 preps-saliva)
Reagent bottle size/volume1 CY1: 60mL
Reagent bottle size/volume2 CY2: 125mL
Reagent bottle size/volume3 CY3: 125mL
Reagent bottle size/volume4 CY4: 125mL
Reagent bottle size/volume5 CY5: 15mL

 

Product Usage:
Nucleic acid extraction or purification reagents are used for nucleic acid extraction, enrichment, purification and other steps. The processed product is used for clinical in vitro detection.

 

Product Instructions:

1. Before using nucleic acid extraction reagents

①. Transfer proteinase k solvent to lyophilized powder containing proteinase k and mix well.
②Add 18ml and 42ml of absolute ethanol to CY3 and CY4 of CY-F006-10 (50preps-cells) and CY-F006-20 (50preps-saliva), stir well.
③. Add 36ml and 84ml of absolute ethanol to CY3 and CY4 of models CY-F006-11 (100preps-cells) and CY-F006-21 (100preps-saliva) and mix well.

 

2. Swab extraction steps:

①Dry swab collection, add 0.6ml CY1 solution and 10ul proteinase K, mix well, and incubate in a 65°C air incubator for 30 minutes (or wet collection: centrifuge the sample centrifuge tube containing the swab and preservation solution to 12000 Transfer for 1 minute, keep the precipitate, remove the supernatant, add 0.6ml of CY1 solution, 10ul of proteinase K, mix well, and incubate in an air incubator at 65 degrees Celsius for 30 minutes).
②. Remove the swab and centrifuge at 12000rpm for 1 minute.
③. Remove all supernatants into new centrifuge tubes and perform experiments.
④. Add 0.25ml of CY2 solution, 10ul of magnetic beads* (shake well before use), mix for 12min, put it on a magnetic stand for 30s, and blot dry.
⑤ Add 0.6ml of CY3 solution, stir for 3min, put it on a magnetic stand for 30s, and absorb the liquid.
⑥. Add 0.6ml of CY4 solution, mix for 3min, put it on a magnetic stand for 30s, and dry the liquid
⑦, repeat steps ②⑥
⑧ Dry at room temperature for 10-20min, add 50ul CY5 liquid for elution, mix well, place it on a magnetic stand for 30s, and then transfer the liquid to a new centrifuge tube
⑨, measure the outer diameter

 

3. Saliva Extraction Step

① Centrifuge saliva plus preservation solution at 12000rpm for 1min
② Retain the precipitate and remove the supernatant
③. Add 0.6ml of CY1 solution and 10ul of proteinase k, stir evenly, and incubate in an air incubator at 65 degrees Celsius for 30 minutes.
④ Centrifuge at 12000rpm for 1min, take out all the supernatant into a new centrifuge tube, add 10ul of magnetic beads and 0.25ml of CY2, mix for 12min, and place it on a magnetic stand for 30s to absorb the liquid.
⑤ Add 0.6ml of CY3 solution, stir for 3min, put it on a magnetic stand for 30s, and absorb the liquid.
⑥. Add 0.6 ml of CY4 solution, mix for 3 min, place it on a magnetic stand for 30 s, and absorb the liquid.
⑦, repeat step ⑥
⑧ Dry at room temperature for 10-20 minutes, add 50ul CY5 liquid for elution, mix well, put it on a magnetic stand for 30s, and then transfer the liquid to a new centrifuge tube.
⑨, measure the outer diameter

 

Note: To remove RNA, prepare RNaseA 10mg/ml: solvent (10mM sodium acetate: pH 5.0), boil for 15min, adjust pH 7.5 with Tris-HCl, and store at -20 degrees Celsius.

 

FAQ:
Q1: How long have you been in manufacturing medical consumables?

A: Established on 2008, our factory offer production, sales,delivery one-stop service.
Q2: What's your lead time?

A: For mass production, we deliver by batches and first batch can be delivered within 7 days.
Q3: Do you have any certificates?
A: YES, we have all the certificates required for export and local import custom clearance.
Q4: Do you have GMP Lab?

A: YES, we have our GMP Lab built in 2016.
Q5: Where is your main market located?
A: 60% in American Countries, 30% in European Countries and 10% in Middle East.
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Disposable DNA Extraction Kits PCR Viral Nucleic Acid Extraction KitDisposable DNA Extraction Kits PCR Viral Nucleic Acid Extraction Kit

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