DMEM Cell Culture Medium Dulbecco’S Modified 2x Amino Acid Cell Culture Medium

Cell Culture Medium

DMEM Cell Culture Medium Dulbecco's Modified 2x Amino Acid Cell Culture Medium

Description of cell culture medium:

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM culture medium. Compared with MEM culture medium, the content of amino acids has increased by 2 times, the content of vitamins has increased by 4 times, and non-essential amino acids, trace iron ions and sodium pyruvate have also been added. DMEM medium was initially designed with a glucose content of 1000mg/L (low sugar type), and later developed a glucose content of 4500mg/L (high sugar type)

Application of cell culture fluid:

Cell culture medium has been widely used in the cultivation of various cells.
For a wide range of cell culture applications, we offer modified DMEM media in a variety of compositions.

Advantages of cell culture medium:

High-glucose medium is widely used in fast-growing, low-adhesion cells, hybridoma myeloma cells, cloned cells, DNA transfection transformed cells, various primary virus host cells, single cell culture, vaccine production, etc. Production, such as the use of CHO cells to express EPO, to produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells. The sugar-free medium can adjust the glucose concentration at will according to the research needs, which is convenient and quick.

Cell culture fluid composition:

L-Glutamine (Glutamine)

It is an essential nutrient element in cell culture, but is unstable in solution and degrades spontaneously. For medium without L-glutamine, the amount of L-glutamine can be adjusted arbitrarily according to research needs. Adding fresh L-glutamine or its substitutes is more conducive to cell growth.

Phenol red

Used as an indicator of the pH value in the medium for continuous monitoring of the pH value of the medium. At low pH, phenol red makes the medium yellow, and at higher pH, it makes the medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free media formulations can interfere with sodium and potassium balance.

Precautions:

This product has been sterilized by filtration. Care should be taken during an aseptic operation to avoid contamination.

Do not freeze or thaw the product to keep the product in optimal condition.

This product is for scientific research or further research use only, not for diagnosis or treatment.