Manually Nucleic Acid Extraction Kit WIth magnetic bead material

Manually Nucleic Acid Extraction Kit WIth Magnetic Bead Material

HCY Nucleic Acid Extraction Kit for Purifying Isolated DNA Extraction Kit

HCY Extraction Kit provides a fast and efficient magnetic bead method to purify and extract DNA (including genome, mitochondrial and viral DNA) from preserved tissues, saliva, body fluids, oral cavity, cervix, skin cells, bacterial cells, etc. Biological samples can be stored for up to 30 days at room temperature in our proprietary storage buffer prior to processing without significant loss of DNA yield or quality (more than 30 days if stored frozen) No phenol/chloroform extraction or alcohol High Precipitation Yield - Obtain high-quality genomic DNA in 15 minutes with an average DNA yield of 8 micrograms per buccal swab. Purified DNA, approximately 20-30 kb, suitable for downstream applications such as PCR or other enzymatic reactions

HCY Extraction Kit Description:

The main ingredients are magnetic beads, proteinase K, and sodium chloride. magnesium chloride. Potassium chloride, Tris, etc.

Advantages of HCY extraction kit:

1. Magnetic bead-based technology isolates genomic DNA without the need for hazardous chemicals, centrifugation or vacuum manifolds, phenol, and ethanol precipitation.

2. Rapid and efficient purification of genomic DNA from human buccal swabs in less than 15 minutes after sample preparation and lysis.

3. Simple cleavage with proteinase K without any mechanical cleavage.

4. Minimal RNA contamination.

5. Purified genomic DNA exhibits improved downstream performance in applications such as PCR.

6. Contains a kit for automated processing of large numbers of samples in 96-well plates using a liquid handling robot.

Product Usage:

For purification and isolation of DNA (including genomic, mitochondrial, bacterial, parasitic and viral DNA) from tissues, saliva, body fluids and oral cavity, cervix, skin cells, bacterial cells, tissue, swabs, cerebrospinal fluid, body fluids, washed urine cells )

Product Instructions:

The magnetic bead material specifically adsorbs and dissociates from nucleic acid under different salt concentrations and acid-base conditions, separates nucleic acid and other components in the lysed cells by the transfer magnetic bead method, and then obtains the magnetic bead-free extract by washing and elution- -- Nucleic acids.

The first step - lysis, use the lysis solution to destroy the cellular structure of animal and plant cells so that the DNA is fully released.

The second step—binding, adding magnetic bead binding solution to specifically adsorb DNA, so that DNA can be fully combined with magnetic beads;

The third step------washing, using detergent, after multiple items of washing, fully removes other residual impurities;

The fourth step - elution, use the eluent to dissociate the magnetic beads and DNA to obtain a higher-quality DNA solution.