DMEM Cell Culture Medium Dulbecco’S Modified 2 Times Amino Acid

DMEM Cell Culture Medium Dulbecco'S Modified 2 Times Amino Acid

DMEM cell culture medium Dulbecco's modified medium 2 times amino acid MEM medium

product description:

ulbecco's modified medium – DMEM (Dulbecco's Modified Eagle Medium) is developed on the basis of MEM medium. Compared with the MEM medium, the content of amino acids has increased by 2 times, the content of vitamins has increased by 4 times, and it has also increased Non-essential amino acids, trace iron ions and sodium pyruvate. DMEM medium was initially designed with a glucose content of 1000mg/L (low sugar type), and later developed a glucose content of 4500mg/L (high sugar type)

Product Usage:

It has been widely used in the culture of various cells.
For a wide range of cell culture applications, we offer modified DMEM media in a variety of components.

Product advantages:

High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells. The glucose-free medium can adjust the concentration of glucose at will according to research needs, which is convenient and quick.

Product ingredients:

L-glutamine (Glutamine)

It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to the growth of cells.

Phenol red

It is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Precautions:

This product has been sterilized by filtration. Care should be taken during an aseptic operation to avoid contamination.

Do not freeze or thaw the product to keep the product in optimal condition.

This product is for scientific research or further research use only, not for diagnosis or treatment.