500ml William’S E Medium Rat Liver Epithelial Cell Hepatocyte Culture Medium

William’s E-Culture Medium

William's E Medium Rat Liver Epithelial Cell Medium Hepatocyte Culture Contains Amino Acids and Vitamins

product description:

William’s E medium was designed by William and Gunn. It is mainly used for the long-term culture of rat liver epithelial cells, and can also be used for the culture of other mammalian liver cells.

Product ingredients:

This product contains amino acids, vitamins, inorganic salts, and other ingredients required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product should be used with serum or serum-free supplements.

L-alanyl-L-glutamine (Alanyl-glutamine, Ala-Glu), also known as alanyl-glutamine, alanyl-glutamine, is an advanced cell culture additive that can directly replace L-glutamine in cell culture media. glutamine. L-glutamine (Glutamine) is an essential nutrient in cell culture, but it is unstable in solution and will spontaneously degrade to generate ammonia and pyroglutamic acid, of which ammonia is harmful to cells; and L-alanyl -L-Glutamine is very stable in aqueous solution and will not degrade spontaneously. The mechanism used by cells is: during cell culture, cells will gradually release a peptidase into the culture medium to hydrolyze L-alanyl-L-glutamyl into L-alanine and L-glutamine, Cells then take up these two hydrolysates. The process of cell utilization of L-alanyl-L-glutamine is similar to the fed-batch culture strategy, in which low-concentration levels of L-glutamine are continuously added to the culture medium, thereby improving the utilization of L-glutamine rate, and will not generate excess ammonia, which is more conducive to cell growth. L-alanyl-L-glutamyl can replace equimolar L-glutamine, is suitable for all cells, has almost no adaptation, and can prolong the culture time of cells and reduce the number of passages, which saves time and money. Activity decreased more slowly compared to cells cultured in a medium supplemented with L-glutamine. The slightly longer lag period is due to the time required for the release of the peptidase and the digestion of the dipeptide.

Phenol red is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.