Medical Nucleic Acid PCR Dna Purification With Magnetic Beads

Medical Nucleic Acid PCR Dna Purification With Magnetic Beads

Magnetic Beads DNA/RNA Purification Kits for PCR Applications Medical Nucleic Acid Purification Kits

product description:

HUACHENYANG DNA Extraction Kit provides a fast and efficient magnetic bead method to purify and extract DNA (including genomic, mitochondrial and viral DNA) from preserved tissues, saliva, body fluids, as well as from oral cavity, cervix, skin cells, bacterial cells, etc. Biological samples can be stored at room temperature for up to 30 days in our proprietary storage buffer before processing without significant loss of DNA yield or quality (more than 30 days if stored frozen) No phenol/ Chloroform extraction or alcohol precipitation yields high-quality genomic DNA within 15 minutes, with an average DNA yield of 8 μg per buccal swab. Purified DNA, approximately 20-30 kb, suitable for downstream applications such as PCR or other enzymatic reactions

Product Usage:

Nucleic acid extraction or purification reagents are used for nucleic acid extraction, enrichment, purification and other steps. The processed product is used for clinical in vitro detection.

Product Instructions:

1. Before using nucleic acid extraction reagents

①. Transfer proteinase k solvent to lyophilized powder containing proteinase k and mix well.
②Add 18ml and 42ml of absolute ethanol to CY3 and CY4 of CY-F006-10 (50preps-cells) and CY-F006-20 (50preps-saliva), stir well.
③. Add 36ml and 84ml of absolute ethanol to CY3 and CY4 of models CY-F006-11 (100preps-cells) and CY-F006-21 (100preps-saliva) and mix well.

2. Swab extraction steps:

①Dry swab collection, add 0.6ml CY1 solution and 10ul proteinase K, mix well, and incubate in a 65°C air incubator for 30 minutes (or wet collection: centrifuge the sample centrifuge tube containing the swab and preservation solution to 12000 Transfer for 1 minute, keep the precipitate, remove the supernatant, add 0.6ml of CY1 solution, 10ul of proteinase K, mix well, and incubate in an air incubator at 65 degrees Celsius for 30 minutes).
②. Remove the swab and centrifuge at 12000rpm for 1 minute.
③. Remove all supernatants into new centrifuge tubes and perform experiments.
④. Add 0.25ml of CY2 solution, 10ul of magnetic beads* (shake well before use), mix for 12min, put it on a magnetic stand for 30s, and blot dry.
⑤ Add 0.6ml of CY3 solution, stir for 3min, put it on a magnetic stand for 30s, and absorb the liquid.
⑥. Add 0.6ml of CY4 solution, mix for 3min, put it on a magnetic stand for 30s, and dry the liquid
⑦, repeat steps ②⑥
⑧ Dry at room temperature for 10-20min, add 50ul CY5 liquid for elution, mix well, place it on a magnetic stand for 30s, and then transfer the liquid to a new centrifuge tube
⑨, measure the outer diameter

3. Saliva Extraction Step

① Centrifuge saliva plus preservation solution at 12000rpm for 1min
② Retain the precipitate and remove the supernatant
③. Add 0.6ml of CY1 solution and 10ul of proteinase k, stir evenly, and incubate in an air incubator at 65 degrees Celsius for 30 minutes.
④ Centrifuge at 12000rpm for 1min, take out all the supernatant into a new centrifuge tube, add 10ul of magnetic beads and 0.25ml of CY2, mix for 12min, and place it on a magnetic stand for 30s to absorb the liquid.
⑤ Add 0.6ml of CY3 solution, stir for 3min, put it on a magnetic stand for 30s, and absorb the liquid.
⑥. Add 0.6 ml of CY4 solution, mix for 3 min, place it on a magnetic stand for 30 s, and absorb the liquid.
⑦, repeat step ⑥
⑧ Dry at room temperature for 10-20 minutes, add 50ul CY5 liquid for elution, mix well, put it on a magnetic stand for 30s, and then transfer the liquid to a new centrifuge tube.
⑨, measure the outer diameter

Note: To remove RNA, prepare RNaseA 10mg/ml: solvent (10mM sodium acetate: pH 5.0), boil for 15min, adjust pH 7.5 with Tris-HCl, and store at -20 degrees Celsius.

Storage conditions and validity period:

1. The product should be used in an environment that is clean and hygienic, avoids pollution, and has a good use environment.
suitable temperature.

2. Store at room temperature. Proteinase K and magnetic beads can be stored longer at 2-8°C.

3. Product shelf life: 12 months