Waymouth Cell Culture Medium For Many Cell Lines Contains Phenol Red

Waymouth Cell Culture Medium For Many Cell Lines Contains Phenol Red

Waymouth’s MB 752/1 medium Chemically defined medium Cell culture medium for many cell lines Contains phenol red​

product description:

This product contains amino acids, vitamins, inorganic salts, and other ingredients required for various types of cell culture, but does not contain proteins, lipids, or any growth factors. Some cells may need to be used with serum.

product description:

Waymouth’s MB 752/1 medium is a chemically defined medium developed by Charity Waymouth, which was originally used to study the nutritional, metabolic, and growth characteristics of the mouse L strain subline L929 cells under serum-free conditions. Waymouth’s MB 752/1 medium has been expanded for use in whole organs as well as in many other cell lines, such as cancer cell lines.

Product ingredients:

L-glutamine (Glutamine)

It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to the growth of cells.

glucose

The concentration of glucose can be adjusted at will according to the needs of the research, which is convenient and quick. High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells.

Phenol red

It is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Precautions:

This product has been sterilized by filtration. Care should be taken during an aseptic operation to avoid contamination.

Do not freeze or thaw the product to keep the product in optimal condition.

This product is for scientific research or further research use only, not for diagnosis or treatment.